Restriction

Restriction may refer to:

  • Restriction (mathematics), an aspect of a mathematical function
  • Restrictions (album), an album by Cactus
  • Restriction enzyme, a type of enzyme that cleaves genetic material
  • Restriction, a term in medieval Supposition theory

Other articles related to "restriction":

PUC19 - Components
6–7 of lac Z are replaced by MCS), where various restriction sites for many restriction endonucleases are present ... The recognition sites for HindIII, SphI, PstI, SalI, XbaI, BamHI, SmaI, KpnI, SacI and EcoRI restriction enzymes have been derived from the vector M13mp19 ...
Bgl II
... BglII (pronounced "bagel two") is a type II restriction endonuclease enzyme isolated from certain strains of Bacillus globigii ... The principal function of restriction enzymes is the protection of the host genome against foreign DNA, but they may also have some involvement in recombination and ... Like most type II restriction enzymes, BglII consists of two identical subunits that form a homodimer around the DNA double helix ...
Amplified Ribosomal DNA Restriction Analysis - ARDRA Rationale and Procedure
... Based on the simple formula for the frequency of random occurrence of a Restriction site, a 4-bp sequence can occur once every 256bp ... A restriction enzyme having a recognition site of more than 4bp would be irrelevant with respect to a gene of approximately 1500bp such as that coding for the 16s ribosomal subunit ... A number of tetracutter Restriction enzymes are available in the market ...
Circularized Chromosome Conformation Capture (4C)
... Step 5a Second Restriction Digest After the reversal of the cross-linked DNA, the restriction fragments are subjected to another round of restriction digest, this ... Step 5c Inverse PCR and Quantitation Primers are designed against the outer restriction sites of the “bait” sequence, which result in the amplification of the small unknown captured fragment ... upstream and downstream regions of all genomic sites of the restriction enzyme used in step 2 ...
Cleaved Amplified Polymorphic Sequence
... It is an extension to the Restriction Fragment Length Polymorphism (RFLP) method, using polymerase chain reaction (PCR) to more quickly analyse the results ... on the principle that genetic differences between individuals can create or abolish restriction endonuclease restriction sites, and that these ... In the CAPS method, PCR amplification is directed across the altered restriction site, and the products digested with the restriction enzyme ...

Famous quotes containing the word restriction:

    If we can find a principle to guide us in the handling of the child between nine and eighteen months, we can see that we need to allow enough opportunity for handling and investigation of objects to further intellectual development and just enough restriction required for family harmony and for the safety of the child.
    Selma H. Fraiberg (20th century)