Western Blot

The western blot (sometimes called the protein immunoblot) is a widely accepted analytical technique used to detect specific proteins in the given sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein.

There are now many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, biochemistry, immunogenetics and other molecular biology disciplines.

Other related techniques include using antibodies to detect proteins in tissues and cells by immunostaining and enzyme-linked immunosorbent assay (ELISA).

The method originated in the laboratory of George Stark at Stanford. The name Western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot.

Read more about Western Blot:  2-D Gel Electrophoresis, Medical Diagnostic Applications, Protocols

Other articles related to "western blot, western, blot":

Lyme Disease - Diagnosis - Laboratory Testing
... most widely available and employed are the Western blot and ELISA ... performed first, and if it is positive or equivocal, then the more specific Western blot is run ... Studies show the Western blot IgM has a specificity of 94–96% for patients with clinical symptoms of early Lyme disease ...
Western Blot - Protocols
... Western Blotting Protocol from Protocolmonkey Modified western blotting protocols from Biotechniques Dr ... Mark Barton Frank Lab protocol Kamps's Western Blotting Protocol http//www.natureprotocols.com/2006/09/15/western_blot_analysis_of_subce.php http//www.prosci-inc.com/Western-Blot-Protocol.html CSH ...
Dot Blot
... A dot blot (or slot blot) is a technique in molecular biology used to detect biomolecules, and for detecting, analyzing, and identifying proteins ... It represents a simplification of the northern blot, southern blot, or western blot methods ... In a dot blot the biomolecules to be detected are not first separated by chromatography ...
Fasciolosis - Diagnosis
... in stool, and immunologically by ELISA and Western blot ... Therefore, immunonological methods such ELISA and enzyme-linked immunoelectrotransfer blot, also called Western blot, are the most important methods in diagnosis of F ... hepatica specific-antibodies are recognized using ELISA or Western blot since 2-4 week post-infection ...
Lyme Disease - Epidemiology - United States
... burgdorferi 2) two-tier testing (ELISA screening and Western blot confirming) or 3) single-tier IgG (old infection) Western blot ... The case definition now includes the use of Western blot without prior ELISA screen ...

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