UFS may refer to:
- Unix File System, a file system used by many Unix and Unix-like operating systems
- United Feature Syndicate, commonly known as United Media
- University of the Free State, a South African university
- Universal Fighting System, a collectible card game
- United Feeder Service, a company formerly part of the United Express carrier network
- Universal Flash Storage
- Universidade Federal de Sergipe, a Brazilian public university based in São Cristóvão, Sergipe
- Unsupervised Forward Selection, a data reduction algorithm
- Universal Frame System, A bolt locking system standard in the construction of aggressive inline skates
- United Family Services