Pfu DNA Polymerase

Pfu DNA Polymerase

Pfu DNA polymerase is an enzyme found in the hyperthermophilic archaeon Pyrococcus furiosus, where it functions to copy the organism's DNA during cell division. In the laboratory setting, Pfu is used to amplify DNA in the polymerase chain reaction (PCR), where the enzyme serves the central function of copying a new strand of DNA during each extension step.

Read more about Pfu DNA Polymerasepolymerase">Proofreading Ability of Pfu Polymerase, History

Other articles related to "pfu dna polymerase, pfu, dna polymerase, dna":

Pfu DNA Polymerase - History
... company Stratagene, based in La Jolla, California, discovered the superiority of Pfu over Taq in 1991 ... "A High Fidelity Thermostable DNA Polymerase Isolated from Pyrococcus Furiosus," Strategies 434-35 (1991) and Gene in December of that year (Gene ... Patent 5,489,523 was granted over exonuclease-deficient Pfu in February 1996 while U.S ...
Royal Jelly - Epigenetic Effects
... In spite of their identical clonal nature at the DNA level, they are strongly differentiated across a wide range of characteristics including anatomical and physiological ... It has been shown that this phenomenon is mediated by an epigenetic modification of DNA known as CpG methylation ... Silencing the expression of an enzyme that methylates DNA in newly hatched larvae led to a royal jelly-like effect on the larval developmental trajectory the majority of individuals with reduced DNA methylation levels ...
History of DNA Research
... Further information History of molecular biology DNA was first isolated by the Swiss physician Friedrich Miescher who, in 1869, discovered a microscopic substance in the pus of discarded surgical bandages ... Levene suggested that DNA consisted of a string of nucleotide units linked together through the phosphate groups ... Astbury produced the first X-ray diffraction patterns that showed that DNA had a regular structure ...
DNA Lounge - History
... by Jim English, Jeff Mason, and Brian Raffi who opened for business under the name DNA Lounge on November 22, 1985 ... Many of DNA's posters and flyers were designed by the San Francisco-based artist, Rex Ray ... During the early 90s, the DNA offered a black membership card for $10 that offered free entry most nights ...
Filter Binding Assay
... affinities between two molecules (often protein and DNA) using a filter ... DNA is negatively charged due to the phosphate backbone and will not "stick" to the nitrocellulose on its own, however, any DNA that has been bound by protein will stick ... The exact amount of DNA "stuck" to the nitrocellulose is quantified by measuring the amount of radioactivity on the filter using a scintillation counter ...

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