Isolation
In 1997, Kennedy from the Keller Lab first isolated the in vitro equivalent of the hemangioblast. These cells were termed blast colony forming cells (BL-CFC). Using aggregates of differentiating mouse embryonic stem cells called embryoid bodies, the authors plated cells in the differentiation timeline just prior to the arise of hematopoietic cells. In the presence of the proper cytokines, a subset of these cells was able to differentiate into hematopoietic lineages. In addition, these same cells can also be differentiated into endothelial cells, as shown by Choi of the Keller Lab.
In 2004, hemangioblasts were isolated in the mouse embryo by Huber of the Keller Lab. They are derived from the posterior primitive streak region of the mesoderm in the gastrulating embryo. By using limiting dilutions, the authors demonstrated that the resulting hematopoietic and endothelial cells were indeed of clonal origin, proving that they had successfully isolated the hemangioblast in the developing embryo.
Read more about this topic: Hemangioblast
Famous quotes containing the word isolation:
“The one happiness is to shut one’s door upon a little room, with a table before one, and to create; to create life in that isolation from life.”
—Eleonora Duse (1859–1924)
“One of the most significant effects of age-segregation in our society has been the isolation of children from the world of work. Whereas in the past children not only saw what their parents did for a living but even shared substantially in the task, many children nowadays have only a vague notion of the nature of the parent’s job, and have had little or no opportunity to observe the parent, or for that matter any other adult, when he is fully engaged in his work.”
—Urie Bronfenbrenner (b. 1917)
“But your isolation must not be mechanical, but spiritual, that is, must be elevation.”
—Ralph Waldo Emerson (1803–1882)