In 1997, Kennedy from the Keller Lab first isolated the in vitro equivalent of the hemangioblast. These cells were termed blast colony forming cells (BL-CFC). Using aggregates of differentiating mouse embryonic stem cells called embryoid bodies, the authors plated cells in the differentiation timeline just prior to the arise of hematopoietic cells. In the presence of the proper cytokines, a subset of these cells was able to differentiate into hematopoietic lineages. In addition, these same cells can also be differentiated into endothelial cells, as shown by Choi of the Keller Lab.
In 2004, hemangioblasts were isolated in the mouse embryo by Huber of the Keller Lab. They are derived from the posterior primitive streak region of the mesoderm in the gastrulating embryo. By using limiting dilutions, the authors demonstrated that the resulting hematopoietic and endothelial cells were indeed of clonal origin, proving that they had successfully isolated the hemangioblast in the developing embryo.
Read more about this topic: Hemangioblast
Other articles related to "isolation":
... of sickness is solitude." —John Donne Isolation is another prominent theme of the story and results from the breakdown of communication death and the threat of illness unpredictable feelings of ... “She had left her home, finally, because she had come near to killing herself.” A sense of isolation can often accompany an illness, leaving one feeling depressed, misunderstood, helpless, angry, or ashamed ...
... Introversion merely describes the need to socially "recharge in isolation" rather than "recharge with company", which describes Extroversion ... lonely in crowds or gatherings, but feel normal in isolation or perhaps with a few close individuals ... Extroverts may feel more lonely in isolation or with few individuals around ...
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Famous quotes containing the word isolation:
“But your isolation must not be mechanical, but spiritual, that is, must be elevation.”
—Ralph Waldo Emerson (18031882)
“I am greatly pleased with the public, authentic isolation in which we two, you and I, now find ourselves. It is wholly in accord with our attitude and our principles.”
—Karl Marx (18181883)
“One of the most significant effects of age-segregation in our society has been the isolation of children from the world of work. Whereas in the past children not only saw what their parents did for a living but even shared substantially in the task, many children nowadays have only a vague notion of the nature of the parents job, and have had little or no opportunity to observe the parent, or for that matter any other adult, when he is fully engaged in his work.”
—Urie Bronfenbrenner (b. 1917)