Gene Knockout

A gene knockout (abbreviation: KO) is a genetic technique in which one of an organism's genes is made inoperative ("knocked out" of the organism). Also known as knockout organisms or simply knockouts, they are used in learning about a gene that has been sequenced, but which has an unknown or incompletely known function. Researchers draw inferences from the difference between the knockout organism and normal individuals.

The term also refers to the process of creating such an organism, as in "knocking out" a gene. The technique is essentially the opposite of a gene knockin. Knocking out two genes simultaneously in an organism is known as a double knockout (DKO). Similarly the terms triple knockout (TKO) and quadruple knockouts (QKO) are used to describe 3 or 4 knocked out genes, respectively.

Read more about Gene KnockoutMethod, Use

Other articles related to "gene knockout, knockouts, gene, knockout":

Gene Knockout - Use
... Knockouts are primarily used to understand the role of a specific gene or DNA region by comparing the knockout organism to a wildtype with a similar ... Knockouts organisms are also used as screening tools in the development of drugs, to target specific biological processes or deficiencies by using a specific ...
Perlecan - Expression - Expression During Development - Animal Models
... mammalian development is demonstrated by perlecan gene knockout experiments ... Nearly half of all mice in which the perlecan gene has been knocked out (perlecan null mice) die at embryonic day 10.5, when the perlecan gene normally starts to be expressed ... The knockout strategy employed for the first perlecan knockout mouse was a floxing of exon 6 by insertion of a neomycin cassette, and subsequent CRE expression for removal of exon 6 from ...