Contents of TBS-Tween
The following is a sample recipe for TBST:
- 50 mM Tris
- 150 mM NaCl
- 0.05% Tween 20
Adjust pH with HCl to pH 7.6
The simplest way to prepare a TBS-Tween solution is to use TBS-T tablets. They are formulated to give a ready to use TBST solution upon dissolution in 500 ml of deionized water.
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