Nai may refer to:
- Nai (caste), a clan/caste in India
- Nai language, a language of Papua New Guinea
- Nai (album), an album by singer Anna Vissi
- Nai (pan flute), a wind instrument, also known as the Moldavian pan flute
- Nai, Rajasthan, a village in India
- Nai, Iran, a village in Razavi Khorasan Province, Iran
- "Nai" (song), a 2007 CD single by Irini Merkouri
- ney (also ney, nye, nay), a wind instrument, more precisely an end-blown flute
- Nai, a Thai feudal title
NAI may refer to:
- NASA Astrobiology Institute (NAI), an organization supporting research of life in the universe
- National Amusements, Inc, a privately owned media and entertainment company
- National Archives of India (NAI), the official archives of the government of India
- National Association for Interpretation (NAI), a professional organization of interpreters
- Natural Alternatives International, the company which manufactures the nutritional supplement Juice Plus
- Network Access Identifier (NAI), an Internet standard defined in RFC 4282
- Network Advertising Initiative, an industry trade group that develops self-regulatory standards for online advertising
- North Allegheny Intermediate High School
- Network Associates, Inc. (NAI), a corporation now known as McAfee, Inc
- Neutral Atom Imaging, a technique used to image magnetospheric and ionospheric plasma populations
- North Adria Aviation in the ICAO airline designator nomenclature
- Netherlands Architecture Institute, a Dutch architecture institute in Rotterdam
- New Atlantic Initiative, an international nonpartisan organization dedicated to revitalizing and expanding the Atlantic community of democracies
- Non-accidental injury
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