Relevant Biological Context
Proteins interact with different protein partners and other macromolecules to achieve functions that support different functions in cells that support survival of the organism. Identifying these interactions may provide clues to their effects on cell processes. As these interactions can be affected by both the internal environment and external stimuli, studying these interactions in vivo and at endogenous levels, as is recommended in BiFC, provides a physiologically-relevant context from which to draw conclusions about protein interactions.
Read more about this topic: Bimolecular Fluorescence Complementation, Strengths
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