Mechanism of Action
The siDNA family, lead by Dbait consists of 32 base pairs deoxyribonucleotide forming an intramolecular double helix, which mimicks DNA double-strand break lesions. Dbait binds to and hyperactivate DNA-PK, an enzyme involved in DNA breaks signaling and repair. DNA-PK hyperactivation induces pan-nuclear phosphorylation of histone H2AX among all the chromatine. H2AX phosphorylation is the signal, which allows double-strand break repair proteins (from NHEJ and Homologous recombination pathways) to form DNA repair complexes selectively on DNA double-strand breaks. Dbait-dependent unspecific phosphorylation of H2AX results in inefficient double strand break recognition and repair.
Read more about this topic: Si DNA
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