Nanopore Sequencing

Nanopore sequencing is a method under development since 1995 for determining the order in which nucleotides occur on a strand of DNA.

A nanopore is simply a small hole, of the order of 1 nanometer in internal diameter. Certain porous transmembrane cellular proteins act as nanopores, and nanopores have also been made by etching a somewhat larger hole (several tens of nanometers) in a piece of silicon, and then gradually filling it in using ion-beam sculpting methods which results in a much smaller diameter hole: the nanopore. Graphene is also being explored as a synthetic substrate for solid-state nanopores.

The theory behind nanopore sequencing is that when a nanopore is immersed in a conducting fluid and a potential (voltage) is applied across it, an electric current due to conduction of ions through the nanopore can be observed. The amount of current is very sensitive to the size and shape of the nanopore. If single nucleotides (bases), strands of DNA or other molecules pass through or near the nanopore, this can create a characteristic change in the magnitude of the current through the nanopore.

Read more about Nanopore SequencingBackground, Fluorescence, Electron Tunneling, Challenges, Commercialization

Other articles related to "nanopore sequencing, nanopores, nanopore":

Nanopore Sequencing - Commercialization
... Agilent Laboratories was the first to license and develop nanopores but does not have any current disclosed research in the area ... The company Oxford Nanopore Technologies in 2008 licensed technology from Harvard, UCSC and other universities and is developing protein and solid state nanopore technology with the ... working device in February 2012 Sequenom licensed nanopore technology from Harvard in 2007 using an approach that combines nanopores and fluorescent labels ...