Knockout Mouse

A knockout mouse is a genetically engineered mouse in which researchers have inactivated, or "knocked out," an existing gene by replacing it or disrupting it with an artificial piece of DNA. The loss of gene activity often causes changes in a mouse's phenotype, which includes appearance, behavior and other observable physical and biochemical characteristics.

Knockout mice are important animal models for studying the role of genes which have been sequenced but whose functions have not been determined. By causing a specific gene to be inactive in the mouse, and observing any differences from normal behaviour or physiology, researchers can infer its probable function.

Mice are currently the most closely related laboratory animal species to humans for which the knockout technique can easily be applied. They are widely used in knockout experiments, especially those investigating genetic questions that relate to human physiology. Gene knockout in rats is much harder and has only been possible since 2003.

The first recorded knockout mouse was created by Mario R. Capecchi, Martin Evans and Oliver Smithies in 1989, for which they were awarded the 2007 Nobel Prize in Physiology or Medicine. Aspects of the technology for generating knockout mice, and the mice themselves have been patented in many countries by private companies.

Read more about Knockout MouseUse, Strains, Procedure, Limitations

Other articles related to "knockout mouse, knockouts, mouse":

Knockout Mouse - Limitations
... While knockout mouse technology represents a valuable research tool, some important limitations exist ... About 15 percent of gene knockouts are developmentally lethal, which means that the genetically altered embryos cannot grow into adult mice ... Knocking out a gene also may fail to produce an observable change in a mouse or may even produce different characteristics from those observed in humans in which ...
SGOL2 - Function - Model Organisms
... Sgol2 knockout mouse phenotype Characteristic Phenotype Homozygote viability Normal Fertility Normal Body weight Normal Anxiety Normal Neurological assessment Normal Grip strength Normal Hot plate Normal ... A conditional knockout mouse line, called Sgol2tm1a(EUCOMM)Wtsi was generated as part of the International Knockout Mouse Consortium program — a high-throughput mutagenesis project to generate and distribute ... Using another genetically engineered mouse that lacks Sgol2 function, and siRNA experiments in oocytes, it has been shown that disruption of the mouse SGOL2 does not cause any alteration in sister chromatid ...
ARID4A - Model Organisms
... Arid4a knockout mouse phenotype Characteristic Phenotype Homozygote viability Abnormal Recessive lethal study Abnormal Fertility Normal Body weight Abnormal Anxiety Normal Neurological assessment Normal Grip ... A conditional knockout mouse line, called Arid4atm1a(EUCOMM)Wtsi was generated as part of the International Knockout Mouse Consortium program — a high-throughput ...
SEC16B - Model Organisms
... Sec16b knockout mouse phenotype Characteristic Phenotype Homozygote viability Normal Fertility Normal Body weight Normal Anxiety Normal Neurological assessment Normal Grip strength Normal Hot plate Normal ... A conditional knockout mouse line, called Sec16btm1a(KOMP)Wtsi was generated as part of the International Knockout Mouse Consortium program — a high-throughput mutagenesis ...
RUNX3 - Knockout Mouse
... Runx3 null mouse gastric mucosa exhibits hyperplasia due to stimulated proliferation and suppressed apoptosis in epithelial cells, and the cells are resistant to TGF-beta ...

Famous quotes containing the word mouse:

    A mouse does not run into the mouth of a sleeping cat.
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