In the field of molecular biology, gene expression profiling is the measurement of the activity (the expression) of thousands of genes at once, to create a global picture of cellular function. These profiles can, for example, distinguish between cells that are actively dividing, or show how the cells react to a particular treatment. Many experiments of this sort measure an entire genome simultaneously, that is, every gene present in a particular cell.
DNA Microarray technology measures the relative activity of previously identified target genes. Sequence based techniques, like serial analysis of gene expression (SAGE, SuperSAGE) are also used for gene expression profiling. SuperSAGE is especially accurate and can measure any active gene, not just a predefined set. The advent of next-generation sequencing has made sequence based expression analysis an increasingly popular, "digital" alternative to microarrays called RNA-Seq. However, microarrays are far more common, accounting for 17,000 PubMed articles by 2006.
Read more about Gene Expression Profiling: Background, Comparison To Proteomics, Use in Hypothesis Generation and Testing, Limitations, Validation of High Throughput Measurements, Statistical Analysis, Gene Annotation, Categorizing Regulated Genes, Finding Patterns Among Regulated Genes, Conclusions
Other articles related to "gene, gene expression, expression, expression profiling, gene expression profiling, genes":
... (commonly known as a genome chip, DNA chip or gene array) ... The process of measuring gene expression via cDNA is called expression analysis or expression profiling ... Applications include Application or technology Synopsis Gene expression profiling In an mRNA or gene expression profiling experiment the expression levels of thousands of genes are simultaneously ...
... Expression profiling provides new information about what genes do under various conditions ... In many cases, analyzing expression profiling results takes far more effort than performing the initial experiments ... and exploratory data analysis before publishing their expression profiling results, coordinating their efforts with a bioinformatician or other expert in microarray technology ...
... SuperSAGE is the most advanced derivate of the serial analysis of gene expression technology (SAGE) for the analysis of expressed genes in eukaryotic organisms (gene expression profiling) ... Like in SAGE, a specific tag from each transcribed gene is recovered ... counting as many tags as possible, the transcription profile, stating what gene is transcribed and how often, becomes apparent ...
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