Amplified Ribosomal DNA Restriction Analysis

Amplified Ribosomal DNA Restriction Analysis

Amplified rDNA (Ribosomal DNA) Restriction Analysis is the extension of the technique of RFLP (Restriction Fragment Length Polymorphism) to the gene encoding the small (16s) ribosomal subunit of bacteria. The technique involves an enzymatic amplification using primers directed at the conserved regions at the ends of the 16s gene, followed by digestion using tetracutter Restriction enzymes. The pattern obtained is said to be representative of the species analysed. Patterns obtained from several restriction enzymes can be used to phylogenetically characterize cultured isolates and 16s genes obtained through cloning from community DNA

Read more about Amplified Ribosomal DNA Restriction Analysis:  ARDRA Rationale and Procedure

Other articles related to "amplified ribosomal dna restriction analysis, restriction, ribosomal":

Amplified Ribosomal DNA Restriction Analysis - ARDRA Rationale and Procedure
... Based on the simple formula for the frequency of random occurrence of a Restriction site, a 4-bp sequence can occur once every 256bp ... A restriction enzyme having a recognition site of more than 4bp would be irrelevant with respect to a gene of approximately 1500bp such as that coding for the 16s ribosomal subunit ... A number of tetracutter Restriction enzymes are available in the market ...

Famous quotes containing the words analysis, restriction and/or dna:

    Ask anyone committed to Marxist analysis how many angels on the head of a pin, and you will be asked in return to never mind the angels, tell me who controls the production of pins.
    Joan Didion (b. 1934)

    If we can find a principle to guide us in the handling of the child between nine and eighteen months, we can see that we need to allow enough opportunity for handling and investigation of objects to further intellectual development and just enough restriction required for family harmony and for the safety of the child.
    Selma H. Fraiberg (20th century)

    Here [in London, history] ... seemed the very fabric of things, as if the city were a single growth of stone and brick, uncounted strata of message and meaning, age upon age, generated over the centuries to the dictates of some now all-but-unreadable DNA of commerce and empire.
    William Gibson (b. 1948)