Neutrophil Cytosolic Factor 4

Neutrophil Cytosolic Factor 4

Identifiers Symbols NCF4; NCF; P40PHOX; SH3PXD4 External IDs OMIM: 601488 MGI: 109186 HomoloGene: 525 GeneCards: NCF4 Gene

Gene Ontology
Molecular function	• protein binding • phosphatidylinositol binding • protein dimerization activity
Cellular component	• cytosol • membrane • phagolysosome • NADPH oxidase complex
Biological process	• antigen processing and presentation of peptide antigen via MHC class I • antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent • immune response • cell communication • antigen processing and presentation of exogenous peptide antigen via MHC class I • interaction with host • oxidation-reduction process • phagosome maturation
Sources: Amigo / QuickGO

RNA expression pattern More reference expression data Orthologs Species Human Mouse Entrez 4689 17972 Ensembl ENSG00000100365 ENSMUSG00000071715 UniProt Q15080 P97369 RefSeq (mRNA) NM_000631 NM_008677 RefSeq (protein) NP_000622 NP_032703 Location (UCSC) Chr 22:
37.26 – 37.27 Mb Chr 15:
78.24 – 78.26 Mb PubMed search

Neutrophil cytosol factor 4 is a protein that in humans is encoded by the NCF4 gene.

The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed. GWAS studies showed that Crohn`s disease patient with certain SNPs in NCF4 are more susceptible to get Crohn`s disease. Crohn`s patient with rs4821544 variants showed a decreased Reactive oxygen species after stimulation with GM-CSF which is a proinflammtory cytokine