Maldi

Matrix-assisted Laser Desorption/ionization - Mass Spectrometer
... The type of a mass spectrometer most widely used with MALDI is the TOF (time-of-flight mass spectrometer), mainly due to its large mass range ... The TOF measurement procedure is also ideally suited to the MALDI ionization process since the pulsed laser takes individual 'shots' rather than working in continuous operation ... MALDI-TOF instrument or reflectron is equipped with an "ion mirror" that reflects ions using an electric field, thereby doubling the ion flight path and increasing the resolution ...

Matrix-assisted Laser Desorption/ionization - Reproducibility and Performance
... The sample preparation for MALDI is important for both sensitivity, reproducibility, and quantification of mass analysis ... phase extraction or by washing the dried-droplet MALDI spots with cold water ... the end of laser pulse and start of ion acceleration in the ion source (in vacuum MALDI sources), laser wavelength, UV energy (as well as its density and homogeneity)in a focused light spot ...

Lipidomics - Experimental Techniques - Lipid Detection - MALDI MS
... MALDI mass spectrometry is a laser-based soft-ionization method often used for analysis of large proteins, but has been used successfully for lipids ... MALDI-Time-of-flight (MALDI-TOF) MS has become a very promising approach for lipidomics studies, particularly for the imaging of lipids from tissue slides ...

Sample Preparation In Mass Spectrometry - Matrix-assisted Laser Desorption/ionization
... MALDI matrices used for identifying peptides include CHCA and DHB ... is mixed in a 11 ratio and then a half a microliter to a microliter is added to the MALDI plate ...

MALDI Imaging - Applications
... MALDI imaging mass spectrometry involves the visualization of the spatial distribution of proteins, peptides, drug candidate compounds and their metabolites, biomarkers or other chemicals within thin slices of sample ... tissue slices mounted on microscope slides and apply a suitable MALDI matrix to the tissue, either manually or now automatically ... Next, the microscope slide is inserted into a MALDI mass spectrometer ...