DSI is an initialism for the following companies:
- Data Sciences International
 - Dave Smith Instruments, an American synthesizer company
 - DaySmart Software, Inc., a software company
 - Deep Springs International
 - Delphi Schools, Inc.
 - Delphine Software International, a now bankrupt software company
 - Destination Software, Inc., a video game company
 - Distinctive Software, Inc., a former Canadian software house
 - Diversified Specialty Institute Holdings, Inc., a US-based healthcare group
 - DYWIDAG Systems International, an international supplier of ground anchors and post-tensioning systems
 - State Hydraulic Works (Turkey) (Turkish: Devlet Su İşleri (DSİ)), a state agency in Turkey
 
DSI also represents the following institutions:
- Data Storage Institute, a Singaporean national research institute
 - Department of Special Investigation, a Thai government organization for special investigation
 - Deutsche Schule Istanbul, a private high school in Istanbul
 - Dienst Speciale Interventies, a Dutch government organization for counter-terrorism actions
 
DSI can also mean:
- Data storage interrupt, the name used for a segmentation fault on PowerPC-based processors
 - Data Stream Interface, computer network protocol to run Apple Filing Protocol over TCP
 - Dead Space Ignition, a video game midquel to the Dead Space series
 - Death or serious injury
 - Delay slot instruction, a term from computer architecture
 - Depolarization-induced suppression of inhibition, a type of modulation of inhibitory neurotransmission
 - Detective Superintendent, a kind of British police's superintendent rank
 - Deutsches SOFIA Institut, an institute helping develop SOFIA
 - Diffused Surface Illumination, a multi-touch technique using a special acrylic Endlighten that disperses even light supplied by edge lighting the acrylic.
 - Diffusion Spectrum Imaging, a brain scanning method used in MRI Tractography to identify crossing fibers in neural tissue
 - Digital Signal Interface, a protocol for controlling of lighting in buildings
 - Disparity Space Image, a data structure used in computer stereo vision
 - Display Serial Interface, a serial protocol for mobile display devices
 - Diverterless supersonic inlet, a type of jet engine air intake
 - Docking Score Index, a drug testing method
 - DontStayIn, a social networking website
 - Dope Stars Inc., an industrial metal band formed in 2002
 - Nintendo DSi, Nintendo's third iteration of the Nintendo DS handheld video-game console
 
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