Besser is the surname of:
- Chaskel Besser (born 1923), Rabbi from Manhattan
- Joe Besser (1907–1988), American comedian
- Les Besser, founder of Compact Software, the first RF and microwave EDA company
- Matt Besser (born 1967), best known from the sketch comedy show Upright Citizens Brigade
- Robert Dennis Besser, see Robert Denning
- Wilibald Swibert Joseph Gottlieb von Besser (1784–1842), Austrian botanist