Restriction Endonuclease

  • (noun): Any of the enzymes that cut nucleic acid at specific restriction sites and produce restriction fragments; obtained from bacteria (where they cripple viral invaders); used in recombinant DNA technology.
    Synonyms: restriction nuclease, restriction enzyme

Some articles on restriction endonuclease, restriction:

Biomolecular Engineering of Molecules - Recombinant DNA - Method
... a genetic sequence corresponding to the recognition site of a restriction endonuclease, such as EcoR1 ... foreign DNA fragments, which have also been cut with the same restriction endonuclease, have been inserted into host cell, the restriction ...
Trans-Spliced Exon Coupled RNA End Determination (TEC-RED) - TEC-RED Methods
... nine step PCR reaction the cDNAs are concurrently embedded with the BpmI restriction endonuclease site (though any class IIs restriction endonuclease may work) and a biotin label which are present in the primers ... then cleaved 14 bp downstream from the recognition site using BpmI restriction endonuclease and blunt ended with T4 DNA polymerase ... then ligated to adapter DNA, in six separate reactions, containing six different restriction endonuclease recognition sites ...
Bgl II
... BglII (pronounced "bagel two") is a type II restriction endonuclease enzyme isolated from certain strains of Bacillus globigii ... The principal function of restriction enzymes is the protection of the host genome against foreign DNA, but they may also have some involvement in ... Like most type II restriction enzymes, BglII consists of two identical subunits that form a homodimer around the DNA double helix ...

Famous quotes containing the word restriction:

    If we can find a principle to guide us in the handling of the child between nine and eighteen months, we can see that we need to allow enough opportunity for handling and investigation of objects to further intellectual development and just enough restriction required for family harmony and for the safety of the child.
    Selma H. Fraiberg (20th century)