Gram Stain

  • (noun): A staining technique used to classify bacteria; bacteria are stained with gentian violet and then treated with Gram's solution; after being decolorized with alcohol and treated with safranine and washed in water, those that retain the gentian violet are gram-positive and those that do not retain it are gram-negative.
    Synonyms: Gram's method, Gram method, Gram's procedure, Gram's stain

Some articles on stain, gram, gram stain:

List of Pathogenic Bacteria By Clinical Characteristics
... that limits skin exposure to ticks insect repellent Microscopy using Giemsa or Wright stain PCR serology (low precision rate) Brucella abortus Brucella canis Brucella ... control Dark-field microscopy on fresh blood smear (but doesn't stain well) Serologic agglutionation tests Listeria monocytogenes Dairy products ... Further 4 months, combination Isoniazid Rifampicin BCG vaccine Isoniazid Ziehl-Neelsen stain showing acid-fast bacteria Hybridization probes for DNA, succeeded by PCR Culture on Lowenstein-Jensen ...
Gram-negative Bacteria - Importance of The Outer Cell Membrane in Bacterial Classification
... are traditionally divided into two main groups, Gram-positive and Gram-negative, based upon their Gram-stain retention property, this classification system is ambiguous as it can refer to three distinct aspects (st ... The Gram-positive and Gram-negative staining response is also not a reliable characteristic as these two kinds of bacteria do not form phylogenetically coherent groups ... However, although Gram-staining response of bacteria is an empirical criterion, its basis lies in the marked differences in the ultrastructure and chemical composition of the ...
Selenomonad - Gram Stain
... See also Gram stain The family Veillonellaceae was transferred from the order Clostridiales to the new order Selenomonadales in the new class Negativicutes ... the members of the Firmicutes staining positive for the Gram stain and being trivially called "low-GC Gram-positives" (c.f ... Bacterial phyla),members of the Negativicutes stain Gram-negative and possess a double bilayer ...
Septic Arthritis - Diagnosis
... Then the gram stain and cultures are typical of a Neisserian infection ... Diagnosis is by aspiration (giving a turbid, non-viscous fluid), Gram stain and culture of fluid from the joint, as well as tell-tale signs in laboratory testing (such as a highly elevated ... The Gram stain can rule in the diagnosis of septic arthritis however cannot exclude it ...
Gram Staining - Uses - Staining Mechanism
... Gram-positive bacteria have a thick mesh-like cell wall made of peptidoglycan (50-90% of cell envelope), which are stained purple by crystal violet, whereas Gram-negative bacteria have a thinner layer (10% of cell ... There are four basic steps of the Gram stain Applying a primary stain (crystal violet) to a heat-fixed smear of a bacterial culture ... The addition of a mordant, which binds to crystal violet and traps it in the cell (Gram's iodine) Rapid decolorization with alcohol or acetone, and Counterstaining with safranin ...

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