DNA Footprinting

DNA Footprinting

DNA footprinting is a method of investigating the sequence specificity of DNA-binding proteins in vitro. This technique can be used to study protein-DNA interactions both outside and within cells.

The regulation of transcription has been studied extensively, and yet there is still much that is not known. Transcription factors and associated proteins that bind promoters, enhancers, or silencers to drive or repress transcription are fundamental to understanding the unique regulation of individual genes within the genome. Techniques like DNA footprinting will help elucidate which proteins bind to these regions of DNA and unravel the complexities of transcriptional control.

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Other articles related to "dna footprinting, footprinting, dna":

DNA Footprinting - Advanced Applications - Footprinting Coupled With Detection By Capillary Electrophoresis
... To adapt the footprinting technique to updated detection methods, the labelled DNA fragments are detected by a capillary electrophoresis device instead of being run on a polyacrylamide gel ... If the DNA fragment to be analyzed is produced by polymerase chain reaction (PCR), it's is straightforward to couple a fluorescent molecule such as carboxyfluorescein (FAM) to the primers ...

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