TLN may refer to:
- The IATA airport code for Toulon-Hyères Airport in Toulon, France
- Telelatino, Spanish and Italian-language specialty cable channel in Canada, which call themselves "TLN"
- Total Living Network, a US religious television network, which also uses the "TLN" abbreviation
- Top lista nadrealista, a popular comedic show in former Yugoslavia that starred members of a band named Zabranjeno Pušenje.
- Tallinn, Estonia.
- Thermolysin, an enzyme