Tiling Arrays are a subtype of microarray chips. Like traditional microarrays, they function by hybridizing labeled DNA or RNA target molecules to probes fixed onto a solid surface.
Tiling arrays differ from traditional microarrays in the nature of the probes. Instead of probing for sequences of known or predicted genes that may be dispersed throughout the genome, tiling arrays probe intensively for sequences which are known to exist in a contiguous region. This is useful for characterizing regions that are sequenced, but whose local functions are largely unknown. Tiling arrays aid in transcriptome mapping as well as in discovering sites of DNA/protein interaction (ChIP-chip, DamID), of DNA methylation (MeDIP-chip) and of sensitivity to DNase (DNase Chip) and array CGH. In addition to detecting previously unidentified genes and regulatory sequences, improved quantification of transcription products is possible. Specific probes are present in millions of copies (as opposed to only several in traditional arrays) within an array unit called a feature, with anywhere from 10,000 to more than 6,000,000 different features per array. Variable mapping resolutions are obtainable by adjusting the amount of sequence overlap between probes, or the amount of known base pairs between probe sequences, as well as probe length. For smaller genomes such as arabidopsis, whole genomes can be examined. Tiling arrays are a useful tool in genome-wide association studies.
Other articles related to "arrays, array, tiling array, tiling arrays":
... Many types of arrays exist and the broadest distinction is whether they are spatially arranged on a surface or on coded beads The traditional solid-phase array is a collection of orderly ... The alternative bead array is a collection of microscopic polystyrene beads, each with a specific probe and a ratio of two or more dyes, which do not interfere with the fluorescent dyes used on the target sequence ... can be then hybridized to a microarray (such as a tiling array) allowing the determination of protein binding site occupancy throughout the genome ...
... Tiling arrays provide an unbiased tool for investigate protein binding, gene expression and gene structure on a genome-wide scope ... Drawbacks include the cost of tiling array kits ... have fallen in the last several years, the price makes it impractical to use genome-wide tiling arrays for mammalian and other large genomes ...
Famous quotes containing the word array:
“Any one who knows what the worth of family affection is among the lower classes, and who has seen the array of little portraits stuck over a labourers fireplace ... will perhaps feel with me that in counteracting the tendencies, social and industrial, which every day are sapping the healthier family affections, the sixpenny photograph is doing more for the poor than all the philanthropists in the world.”
—Macmillans Magazine (London, September 1871)