Reverse transfection is the invention and development of a microarray-driven gene expression system by Junald Ziauddin and David M. Sabatini in 2001. As DNA are printed on a glass slide for the transfection process (the deliberate introduction of nucleic acids into cells) to occur before the addition of adherent cells, the order of addition of DNA and adherent cells is reverse that of conventional transfection. Hence, the word “reverse” is used.
Read more about Reverse Transfection: Advantages and Disadvantages of Reverse Transfection
Other articles related to "reverse transfection, transfection":
... The advantages of reverse transfection (over conventional transfection) are The addition and attachment of target cells to the DNA-loaded surface can lead to a higher probability of cell-DNA contact, potentially ... printed on different slides for at least 15 months' storage without apparent loss of transfection efficiency ... The disadvantages of reverse transfection are Reverse transfection is more expensive because a highly accurate and efficient microarray printing system is needed to print the DNA-gelatin solution onto the ...
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