Thermal Cycling

Some articles on cycling, thermal, thermal cycling:

Polymerase Chain Reaction - Variations On The Basic PCR Technique
... Assembly PCR or Polymerase Cycling Assembly (PCA) artificial synthesis of long DNA sequences by performing PCR on a pool of long oligonucleotides with short overlapping ... amplification similar to traditional PCR, but uses a constant temperature rather than cycling through denaturation and annealing/extension cycles ... DNA helicase, an enzyme that unwinds DNA, is used in place of thermal denaturation ...
Bio-MEMS For Diagnostics - PCR Chips
... The reaction involves thermal cycling of the DNA sequence and DNA polymerase through three different temperatures ... The advantages of PCR chips include shorter thermal-cycling time, more uniform temperatures during the PCR process for enhanced yield, and portability ... For example, silicon substrates have good thermal conductivity for rapid heating and cooling, but can poison the polymerase reaction ...
Polymerase Chain Reaction
... The method relies on thermal cycling, consisting of cycles of repeated heating and cooling of the reaction for DNA melting and enzymatic replication ... The vast majority of PCR methods use thermal cycling, i.e ... These thermal cycling steps are necessary first to physically separate the two strands in a DNA double helix at a high temperature in a process called DNA melting ...

Famous quotes containing the word cycling:

    From the war of nature, from famine and death, the most exalted object which we are capable of conceiving, namely, the production of the higher animals, directly follows. There is grandeur in this view of life, with its several powers, having been breathed into a few forms or into one; and that, whilst this planet has gone cycling on according to the fixed law of gravity, from so simple a beginning endless forms most beautiful and most wonderful have been, and are being, evolved.
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