In molecular biology, real-time polymerase chain reaction, also called quantitative real time polymerase chain reaction (qPCR) or kinetic polymerase chain reaction is a laboratory technique based on the polymerase chain reaction, which is used to amplify and simultaneously quantify a targeted DNA molecule. For one or more specific sequences in a DNA sample, Real Time-PCR enables both detection and quantification. The quantity can be either an absolute number of copies or a relative amount when normalized to DNA input or additional normalizing genes.
The procedure follows the general principle of polymerase chain reaction; its key feature is that the amplified DNA is detected as the reaction progresses in real time. This is a new approach compared to standard PCR, where the product of the reaction is detected at its end. Two common methods for the detection of products in real-time PCR are: (1) non-specific fluorescent dyes that intercalate with any double-stranded DNA, and (2) sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter which permits detection only after hybridization of the probe with its complementary DNA target.
Frequently, real-time PCR is combined with reverse transcription to quantify messenger RNA (mRNA) and non-coding RNA in cells or tissues.
qPCR is the abbreviation used for real-time PCR. Real-time reverse-transcription PCR is often denoted as: qRT-PCR The acronym "RT-PCR" commonly denotes reverse transcription polymerase chain reaction and not real-time PCR, but not all authors adhere to this convention.
Other articles related to "polymerase, reaction, polymerases, chain":
... The T7 DNA polymerase of the T7 bacteriophage is a DNA-dependent DNA polymerase responsible for the fast rate of T7 phage DNA replication in vivo ... The polymerase consists of a 11 complex of the viral T7 gene 5 protein (80kDA) and the E ... This polymerase is unique due to its considerable processivity, or ability to stay on DNA for a greater than average number of base pairs ...
... This reaction proceeds efficiently when this solution is incubated at room temperature with required salt ... fragments amplified by either Taq or Pfu polymerase as Taq polymerase (unlike Pfu) leaves an extra "A" nucleotide at the 3'end during amplification ... insert is created by PCR using Taq DNA polymerase, a polymerase that lacks 3' to 5' proofreading activity and with a high probability adds a single, 3'-adenine overhang to each end of the ...
... A polymerase is an enzyme whose central biological function is the synthesis of polymers of nucleic acids ... DNA polymerase and RNA polymerase are used to assemble DNA and RNA molecules, respectively, generally by copying a DNA or RNA template strand using base-p ... for the generation of other biopolymers are not also referred to as polymerases ...
... Streptolydigin is an antibiotic which works by blocking nucleic acid chain elongation by binding to the polymerase, thus stopping RNA polymerase activity inside a cell ... Specifically, it inhibits the assembly of the RNA polymerase II transcription complex and DNA polymerase III transcription ...
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