Lactate Dehydrogenase Elevating Virus - Structure

Structure

LDV has a genome that consists of single stranded positive sense RNA that is 14.1kb long. The genome is dominated by two large open reading frames, ORF1a and ORF1ab, these code for two polyproteins, PP1a and PP1ab. These polyproteins are thought to be cleaved into 12 products. The virus contains a nucleocapsid that is spherical with a diameter of 35 ± 4 nm. This is then enclosed in an envelope to create a smooth surface. The envelope consists of two proteins, VP2 and VP3. VP2 has a molecular weight of 18,000, and VP3 is a heterogeneous glycoprotein of molecular weight 15,000. The envelope is extremely labile and tends to slough off; this characteristic is indicated by its extreme sensitivity to detergent treatment. The virus has a density of 1.13g/mL and the nucleocapsid has a density of 1.17g/mL in a sucrose density gradient. LDV has been shown to mature by budding through the intracytoplasmic membrane. The virions have four structural proteins which include a nucleocapsid protein, a non-glycosylated envelope protein, a major envelope glycoprotein, and a minor envelope glycoprotein.

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