Adaptive Mutation

Adaptive mutation is a theoretical evolutionary mechanism. Adaptive mutation proposes that genetic mutations may arise as an immediate and direct response to selective pressures. This is in contrast to mainstream evolutionary theory, which holds that mutagenesis occurs randomly, regardless of the utility of a genetic mutation to the organism, and that mutations with survival advantage are then chosen by natural selection.

In 1988 molecular biologist John Cairns et al. proposed that "when populations of single cells are subject to certain forms of strong selection pressure, variants emerge bearing changes in DNA sequence that bring about an appropriate change in phenotype." This suggests that there exists a particular physiological pathway that responds to a specific selective pressure to produce a mutation conferring the correct phenotype that will alleviate this pressure.

The original Cairns experiments involved a strain of E. coli that has a frameshift mutation in the lactose (LacZ) operon, inactivating the proteins needed for utilization of this sugar. The bacteria were then spread on an agar medium in which the only carbon source was lactose. This meant that a cell could grow only if a second mutation occurred in the lactose operon, reversing the effects of the mutation and therefore allowing the enzymes to be synthesized. Mutations with this effect appeared to occur significantly more frequently than expected, and at a rate that was greater than mutations in other parts of the genomes of these E. coli cells.

There is however a serious flaw in this experiment, as Cairns does not distinguish between selection and detection of LacZ revertants. If he is testing to see if the presence of lactose as the selective agent causes mutations that confer the ability to eat lactose, then he should not detect this mutation with lactose present (i.e. looking for cells that grow with lactose as the only carbon source.) He will never know, in this case, if cells have acquired this ability without the presence of lactose – a possibility that his theory cannot reconcile. In fact, the acknowledgment of fundamental limitations on our ability to separate between mutation selection and detection has led Vasily Ogryzko to suggest that for the proper description of the Cairns' experiments, the formalism of quantum theory would be required, with the phenomenon of adaptive mutations naturally following from such an approach.

Furthermore, when looking for additional mutations only in cells that have already reverted to Lac+, it would certainly be the case that other, unnecessary mutations would be less numerous - especially since most are deleterious. However, Barry Hall has provided evidence that the mutation rate in bacteria under environmental stress increases across the board, most likely indiscriminately. When testing for tryptophan revertants (trp− → trp+, i.e. cells that have regained the ability to make tryptophan), he found that occurrence of auxotrophic mutants increased as well. Tryptophan revertants, which had been exposed to the environmental stress of lacking the amino acid, saw a 1.8% rate of auxotrophy for any other amino acid. When testing for auxotrophy in cells in non-stressed colonies, he found a rate below 0.01%. From these data, Hall hypothesized that cells under stress enter a "hyper-mutable state," where cells increase their general rate of mutation, increasing the overall probability that they will acquire a mutation conferring a phenotype that aids their survival. Hall later determined that his mutants were the result of transposon activity, and concluded that his results were in fact about transposon biology, and not mutagenesis.

Similar results have been observed in other experiments. These experiments suggested that mutations in bacteria are influenced by the selective pressures that the bacteria are placed under.

One possible explanation is that under conditions of stress the global rate of errors in DNA replication and repair mechanisms is increased, and hence the mutation rate is increased.

Another explanation stems from a similarity in cellular mechanisms underlying the acquisition of adaptive mutations in the bacterial stationary phase cells and in the mammalian tumor cells. In both cases the adaptive mutations arise in response to a sustained stress environment and are promoted by high rate of genomic mutations. Cellular processes leading to the mutations are also surprisingly similar between both organisms and include silencing of differentiation, cellular senescence, programmed cell death, and DNA repair on the one hand, and activation of the error-prone replication and transposons on the other. The similarity suggests that the adaptive mutations may be an output of activation in the stressed cell of a special survival strategy for quick adaptation to the stressful environment. This strategy that is also referred as the mutator phenotype is an alternative to other stress-induced strategies, such as senescence and programmed cell death, activated in majority of stressed cells. Continuing stress-induced proliferative and survival signaling may be an important prerequisite for epigenetic reprogramming of some cells to activate the mutator phenotype.

Cairns' observation of apparently adaptive mutations in the lac operon have been shown to have a completely orthodox Darwinian explanation, as explained in the 2002 article by John Roth and colleagues to be found here: http://www.pnas.org/content/99/4/2164.full. The senior author and the contributing editor are world famous scientists and their explanation is entirely credible. Later research published in 2006 by Jeffrey D. Stumpf, Anthony R. Poteete, and Patricia L. Foster, however, concluded that amplification could not account for the adaptive mutation and that "mutants that appear during the first few days of lactose selection are true revertants that arise in a single step".

Other articles related to "adaptive, mutation, mutations, adaptive mutation":

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... cells is based on a mechanism whereby cells individually undergo mutation, with the process of natural selection then culling out those mutations which are less beneficial to ... to provide a theoretical mechanism which would skew these random mutations in favor of some outcome beneficial to the cell ... the outset that this theory would only be useful if indeed there were evidence that some sort of adaptive mutation occurs - in other words, if there were experimental data ...

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