Sticky and Blunt Ends

Sticky And Blunt Ends

DNA end or sticky end refers to the properties of the end of a molecule of DNA or a recombinant DNA molecule. The concept is important in molecular biology, especially in cloning or when subcloning inserts DNA into vector DNA. All the terms can also be used in reference to RNA. The sticky ends or cohesive ends form base pairs. Any two complementary cohesive ends can anneal, even those from two different organisms. This bondage is temporary however, and DNA ligase will eventually form a covalent bond between the sugar-phosphate residue of adjacent nucleotides to join the two molecules together.

Read more about Sticky And Blunt EndsSingle-stranded DNA Molecules, Variations in Double-stranded Molecules, Discovery, References

Famous quotes containing the words ends, sticky and/or blunt:

    More are men’s ends marked than their lives before.
    The setting sun, and music at the close,
    As the last taste of sweets, is sweetest last,
    Writ in remembrance more than things long past.
    William Shakespeare (1564–1616)

    The kiss. There are all sorts of kisses, lad, from the sticky confection to the kiss of death. Of them all, the kiss of an actress is the most unnerving. How can we tell if she means it or if she’s just practicing?
    Ruth Gordon (1896–1985)

    Comedy has to be done en clair. You can’t blunt the edge of wit or the point of satire with obscurity. Try to imagine a famous witty saying that is not immediately clear.
    James Thurber (1894–1961)