A Southern blot is a method routinely used in molecular biology for detection of a specific DNA sequence in DNA samples. Southern blotting combines transfer of electrophoresis-separated DNA fragments to a filter membrane and subsequent fragment detection by probe hybridization. The method is named after its inventor, the British biologist Edwin Southern. Other blotting methods (i.e., western blot, northern blot, eastern blot, southwestern blot) that employ similar principles, but using RNA or protein, have later been named in reference to Edwin Southern's name. As the technique was eponymously named, Southern blot is capitalized as is conventional for proper nouns. The names for other blotting methods may follow this convention, by analogy.
Other articles related to "southern blot, southern, blot":
... Southern transfer may be used for homology-based cloning on the basis of amino acid sequence of the protein product of the target gene ... Second, Southern blotting can also be used to identify methylated sites in particular genes ...
... A Southern blot is a method routinely used in molecular biology for detection of a specific DNA sequence in DNA samples ... Southern blotting combines transfer of electrophoresis-separated DNA fragments to a filter membrane and subsequent fragment detection by probe ...
... The Southern blot is used for DNA analysis and was routinely used for genetic fingerprinting and paternity testing prior to the development of microsatellite markers for this purpose ... The concepts of the Southern blot were used in the development and creation of the modern microarray slide, which is a promising experimental tool ... The northern blot is a similar procedure for RNA, playing off the Southern name ...
Famous quotes containing the words blot and/or southern:
“O to blot out this garden
to forget, to find a new beauty
in some terrible
—Hilda Doolittle (18861961)
“I sometimes wonder that we can be so frivolous ... as to attend to the gross but somewhat foreign form of servitude called Negro Slavery, there are so many keen and subtle masters that enslave both north and south. It is hard to have a southern overseer; it is worse to have a northern one; but worst of all when you are the slave-driver of yourself.”
—Henry David Thoreau (18171862)