Size-exclusion Chromatography

Size-exclusion Chromatography

Size-exclusion chromatography (SEC) is a chromatographic method in which molecules in solution are separated by their size, and in some cases molecular weight. It is usually applied to large molecules or macromolecular complexes such as proteins and industrial polymers. Typically, when an aqueous solution is used to transport the sample through the column, the technique is known as gel-filtration chromatography, versus the name gel permeation chromatography, which is used when an organic solvent is used as a mobile phase. SEC is a widely used polymer characterization method because of its ability to provide good molar mass distribution (Mw) results for polymers.

Read more about Size-exclusion ChromatographyApplications, Advantages, Discovery, Theory and Method, Factors Affecting Filtration, Analysis, Drawback, Absolute Size-exclusion Chromatography

Other articles related to "chromatography":

High-performance Liquid Chromatography - Types - Size-exclusion Chromatography
... For more details on this topic, see size-exclusion chromatography ... Size-exclusion chromatography (SEC), also known as gel permeation chromatography or gel filtration chromatography, separates particles on the basis of molecular size (actually by a particle's Stokes radius) ... It is generally a low resolution chromatography and thus it is often reserved for the final, "polishing" step of a purification ...
Absolute Size-exclusion Chromatography
... Absolute size-exclusion chromatography (ASEC) is a technique that couples a dynamic light scattering (DLS) instrument to a size exclusion chromatography system for ...