SDS-PAGE - Chemical Ingredients and Their Roles

Chemical Ingredients and Their Roles

Polyacrylamide gel (PAG) had been known as a potential embedding medium for sectioning tissues as early as 1964, and two independent groups employed PAG in electrophoresis in 1959. It possesses several electrophoretically desirable features that make it a versatile medium. It is a synthetic, thermo-stable, transparent, strong, chemically relatively inert gel, and can be prepared with a wide range of average pore sizes. The pore size of a gel is determined by two factors, the total amount of acrylamide present (%T) (T = Total concentration of acrylamide and bisacrylamide monomer) and the amount of cross-linker (%C) (C = bisacrylamide concentration). Pore size decreases with increasing %T; with cross-linking, 5%C gives the smallest pore size. Any increase or decrease in %C from 5% increases the pore size, as pore size with respect to %C is a parabolic function with vertex as 5%C. This appears to be because of non-homogeneous bundling of polymer strands within the gel. This gel material can also withstand high voltage gradients, is amenable to various staining and destaining procedures, and can be digested to extract separated fractions or dried for autoradiography and permanent recording.

Read more about this topic:  SDS-PAGE

Other articles related to "chemical ingredients and their roles, chemicals":

SDS-PAGE - Chemical Ingredients and Their Roles - Chemicals For Processing and Visualization
... The following chemicals are used for processing of the gel and the protein samples visualized in it Tracking dye ...

Famous quotes containing the words roles, chemical and/or ingredients:

    It was always the work that was the gyroscope in my life. I don’t know who could have lived with me. As an architect you’re absolutely devoured. A woman’s cast in a lot of roles and a man isn’t. I couldn’t be an architect and be a wife and mother.
    Eleanore Kendall Pettersen (b. 1916)

    We are close to dead. There are faces and bodies like gorged maggots on the dance floor, on the highway, in the city, in the stadium; they are a host of chemical machines who swallow the product of chemical factories, aspirin, preservatives, stimulant, relaxant, and breathe out their chemical wastes into a polluted air. The sense of a long last night over civilization is back again.
    Norman Mailer (b. 1923)

    Copernicanism and other essential ingredients of modern science survived only because reason was frequently overruled in their past.
    Paul Feyerabend (1924–1994)