Protein Structure - Protein Structure Determination

Protein Structure Determination

Around 90% of the protein structures available in the Protein Data Bank have been determined by X-ray crystallography. This method allows one to measure the 3D density distribution of electrons in the protein (in the crystallized state) and thereby infer the 3D coordinates of all the atoms to be determined to a certain resolution. Roughly 9% of the known protein structures have been obtained by Nuclear Magnetic Resonance techniques. The secondary structure composition can be determined via circular dichroism . Cryo-electron microscopy has recently become a means of determining protein structures to high resolution (less than 5 angstroms or 0.5 nanometer) and is anticipated to increase in power as a tool for high resolution work in the next decade. This technique is still a valuable resource for researchers working with very large protein complexes such as virus coat proteins and amyloid fibers. A more qualitative picture of protein structure is often obtained by proteolysis, which is also useful to screen for more crystallisable protein samples. Novel implementations of this approach (including Fast parallel proteolysis (FASTpp)) can probe the structured fraction and its stability without the need for purification.

Read more about this topic:  Protein Structure

Other articles related to "structure":

Book Of Revelation - Content - Literary Structure
... In terms of literary structure, Revelation consists of four visions, each involving John “seeing” the plan of God unveiled, with an epilogue that concludes the book ... In terms of content, the structure of Revelation is built around four successive groups of seven the messages to the seven churches the seven seals the seven trumpets and the seven bowl judgments ... Kenneth Strand asserts that symbols and phrases of Revelation are organized as a chiastic structure, a literary device used frequently in the Old Testament ...
321 Kinematic Structure
321 kinematic structure is a design method for robotic arms (serial manipulators), invented by Donald L ... Many other industrial robots, such as the PUMA, have a kinematic structure that deviates a little bit from the 321 structure ...
Zirconium Tungstate - Cubic Phase
... Since the structure is cubic, as described below, the thermal contraction is isotropic - equal in all directions ... The structure of cubic zirconium tungstate consists of corner-sharing ZrO6 octahedral and WO4 tetrahedral structural units ... of the polyhedral units that make up the structure, and lead to contraction ...
Watchmen - Art and Composition - Structure
... The cover of each issue serves as the first panel to the story ... Gibbons said, "The cover of the Watchmen is in the real world and looks quite real, but it's starting to turn into a comic book, a portal to another dimension." The covers were designed as close-ups that focused on a single detail with no human elements present ...
Book Of Ruth - Structure - Ethical Importance
2 ... Loving-kindness living Boaz and Ruth are models of an altruism for which the word "loving-kindness" has been coined (approximately translating Hebrew hesed) ...

Famous quotes containing the words protein and/or structure:

    Firm-style bean curd insoles cushion feet, absorb perspiration and provide more protein than meat or fish innersoles of twice the weight. Tofu compresses with use, becoming more pungent and flavorful. May be removed when not in use to dry or marinate. Innersoles are ready to eat after 1,200 miles of wear. Each pair provides adult protein requirement for 2 meals. Insoles are sized large to allow for snacks. Recipe booklet included.
    Alfred Gingold, U.S. humorist. Items From Our Catalogue, “Tofu Innersoles,” Avon Books (1982)

    ... the structure of our public morality crashed to earth. Above its grave a tombstone read, “Be tolerant—even of evil.” Logically the next step would be to say to our commonwealth’s criminals, “I disagree that it’s all right to rob and murder, but naturally I respect your opinion.” Tolerance is only complacence when it makes no distinction between right and wrong.
    Sarah Patton Boyle, U.S. civil rights activist and author. The Desegregated Heart, part 2, ch. 2 (1962)