Polymerase Chain Reaction Inhibitors

Polymerase Chain Reaction Inhibitors

PCR inhibitors are any factor which prevent the amplification of nucleic acids through the polymerase chain reaction (PCR). PCR inhibition is the most common cause of amplification failure when sufficient copies of DNA are present. PCR inhibitors usually affect PCR through interaction with DNA or interference with the DNA polymerase. Inhibitors can escape removal during the DNA purification procedure by binding directly to single or double-stranded DNA. Alternatively, by reducing the availability of cofactors (such as Mg2+) or otherwise interfering with their interaction with the DNA polymerase, PCR is inhibited.

In a multiplex PCR reaction, it is possible for the different sequences to suffer from different inhibition effects to different extents, leading to disparity in their relative amplifications.

Read more about Polymerase Chain Reaction InhibitorsTypes of Inhibitors, Quantifying Extent of Inhibition, See Also

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Polymerase Chain Reaction Inhibitors - See Also
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