Polymerase Chain Reaction Inhibitors
PCR inhibitors are any factor which prevent the amplification of nucleic acids through the polymerase chain reaction (PCR). PCR inhibition is the most common cause of amplification failure when sufficient copies of DNA are present. PCR inhibitors usually affect PCR through interaction with DNA or interference with the DNA polymerase. Inhibitors can escape removal during the DNA purification procedure by binding directly to single or double-stranded DNA. Alternatively, by reducing the availability of cofactors (such as Mg2+) or otherwise interfering with their interaction with the DNA polymerase, PCR is inhibited.
In a multiplex PCR reaction, it is possible for the different sequences to suffer from different inhibition effects to different extents, leading to disparity in their relative amplifications.
Other articles related to "polymerase chain reaction inhibitors":
... PCR optimisation PCR inhibitor page at OpenWetWare, the life scientist wiki. ...
Famous quotes containing the words reaction and/or chain:
“Christianity was only a very strong and singularly well-timed Salvation Army movement that happened to receive help from an unusual and highly dramatic incident. It was a Puritan reaction in an age when, no doubt, a Puritan reaction was much wanted; but like all sudden violent reactions, it soon wanted reacting against.”
—Samuel Butler (18351902)
“Loyalty to petrified opinions never yet broke a chain or freed a human soul in this worldand never will.”
—Mark Twain [Samuel Langhorne Clemens] (18351910)