Overlap Extension Polymerase Chain Reaction

The overlap extension polymerase chain reaction (or OE-PCR) is a variant of PCR. It is also referred to as Splicing by overlap extension / Splicing by overhang extension (SOE) PCR. It is used to insert specific mutations at specific points in a sequence or to splice smaller DNA fragments into a larger polynucleotide.

Read more about Overlap Extension Polymerase Chain Reaction:  Splicing of DNA Molecules, Introduction of Mutations

Other articles related to "overlap extension polymerase chain reaction, reaction, overlap extension, overlap, extension, polymerase, polymerases, chain":

Overlap Extension Polymerase Chain Reaction - Introduction of Mutations
... The replication reaction continues to produce a fully dimerised DNA fragment ... a 110 nt primer will allow, it is necessary to employ overlap extension PCR ... Only the duplex without overlap at the 5' end will allow extension by DNA polymerase in 3' to 5' direction ...
T7 DNA Polymerase
... The T7 DNA polymerase of the T7 bacteriophage is a DNA-dependent DNA polymerase responsible for the fast rate of T7 phage DNA replication in vivo ... The polymerase consists of a 11 complex of the viral T7 gene 5 protein (80kDA) and the E ... This polymerase is unique due to its considerable processivity, or ability to stay on DNA for a greater than average number of base pairs ...
TOPO Cloning - Technique
... This reaction proceeds efficiently when this solution is incubated at room temperature with required salt ... types of vectors are used for cloning fragments amplified by either Taq or Pfu polymerase as Taq polymerase (unlike Pfu) leaves an extra "A" nucleotide at the 3'end during ... The insert is created by PCR using Taq DNA polymerase, a polymerase that lacks 3' to 5' proofreading activity and with a high probability adds a single, 3'-adenine overhang to each end of the PCR product ...
... A polymerase is an enzyme whose central biological function is the synthesis of polymers of nucleic acids ... DNA polymerase and RNA polymerase are used to assemble DNA and RNA molecules, respectively, generally by copying a DNA or RNA template strand using base-pairing interactions ... for the generation of other biopolymers are not also referred to as polymerases ...
... which works by blocking nucleic acid chain elongation by binding to the polymerase, thus stopping RNA polymerase activity inside a cell ... Specifically, it inhibits the assembly of the RNA polymerase II transcription complex and DNA polymerase III transcription ...

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