Oligonucleotides

Some articles on oligonucleotides, oligonucleotide:

Artificial Gene Synthesis - Standard Methods - Limitations
... specific alignment of long single stranded oligonucleotides, critical parameters for synthesis success include extended sequence regions comprising secondary structures caused by inverted repeats ... gene synthesis experiment depends strongly on the quality of the oligonucleotides used ... on the correctness of the employed oligonucleotides ...
Bio-MEMS For Diagnostics - Genomic and Proteomic Microarrays - Oligonucleotide Chips
... Oligonucleotide chips are microarrays of oligonucleotides ... The main methods for creating an oligonucleotide microarray are by gel pads (Motorola), microelectrodes (Nanogen), photolithography (Affymetrix), and inkjet technology (Agilent) ... Using gel pads, prefabricated oligonucleotides are attached to patches of activated polyacrylamide Using microelectrodes, negatively charged DNA and molecular probes can be concentrated on energized ...
Oligonucleotide Synthesis
... Oligonucleotide synthesis is the chemical synthesis of relatively short fragments of nucleic acids with defined chemical structure (sequence) ... and inexpensive access to custom-made oligonucleotides of the desired sequence ... Whereas enzymes synthesize DNA and RNA in a 5' to 3' direction, chemical oligonucleotide synthesis is carried out in the opposite, 3' to 5' direction ...
Delitto Perfetto - General Considerations For Oligonucleotide Design
80-100 bp oligonucleotides can be generated as single molecules, or pairs of oligonucleotides that are completely overlapping or partially overlapping ... The type of oligonucleotide recommended depends on the type of mutation and the distance from the CORE cassette integration site a mutation is desired ... Longer oligonucleotides lead to increased transformation efficiency ...
Computational Gene - Challenges
... signals can be irreversibly linked to one end of the oligonucleotides, forming an oligonucleotide-peptide conjugate that allows effective internalisation of DNA into the ... nuclease sensitivity include modifications of the oligonucleotide backbone such as methylphosphonate and phosphorothioate (S-ODN) oligodeoxynucleotides, but along with their increased stability, modified ... In vivo applications of antisense oligonucleotides showed that toxicity is largely due to impurities in the oligonucleotide preparation and lack of specificity of the ...