Lysates

Some articles on lysate, lysates:

Protein Microarray - Types of Array
... probed with a complex protein solution such as a cell lysate ... the response of the cells to a particular factor can be identified by comparing the lysates of cells treated with specific substances or grown under certain ... (RPA) These arrays involve complex samples, such as tissue lysates ...
Reverse Phase Protein Lysate Microarray
... Technically, minuscule amounts of a) cellular lysates, from intact cells or laser capture microdissected cells, b)body fluids such as serum, CSF, urine, vitreous, saliva, etc ... Multiplexing is achieved by probing multiple arrays spotted with the same lysate with different antibodies simultaneously and can be implemented as a quantitative calibrated assay ... In addition, since RPMA can utilize whole-cell or undissected or microdissected cell lysates, it can provide direct quantifiable information concerning post translationally modified proteins that are ...
Reverse Phase Protein Lysate Microarray - Experimental Design and Procedure - RPMA Construction
... Cell lysates are collected and are serially diluted six to ten times if using colorimetric techniques, or without dilution when fluorometric detection is used (d ... The lysates are then printed onto either nitrocellulose or PVDF membrane coated glass slides by a microarrayer such as Aushon BioSystem 2470 or Flexys robot (Genomic solution) ... ideal choice as it can be used for producing arrays with very viscous lysates and it has humidity environmental control and automated slide supply system ...