Lysate

Some articles on lysate, lysates:

Reverse Phase Protein Lysate Microarray
... Technically, minuscule amounts of a) cellular lysates, from intact cells or laser capture microdissected cells, b)body fluids such as serum, CSF, urine, vitreous, saliva, etc ... Multiplexing is achieved by probing multiple arrays spotted with the same lysate with different antibodies simultaneously and can be implemented as a quantitative ... RPMA can utilize whole-cell or undissected or microdissected cell lysates, it can provide direct quantifiable information concerning post translationally modified proteins ...
Immunoprecipitation - Technological Advances - Agarose - Preclearing
... Lysates are complex mixtures of proteins, lipids, carbohydrates and nucleic acids, and one must assume that some amount of non-specific binding to the IP antibody, Protein A/G or the ... In most cases, preclearing the lysate at the start of each immunoprecipitation experiment (see step 2 in the "protocol" section below) is a way to remove potentially reactive ... The basic preclearing procedure is described below, wherein the lysate is incubated with beads alone, which are then removed and discarded prior to the immunoprecipitation This approach, though, does not ...
Crude Lysate
... A crude lysate is the solution produced when cells are destroyed by disrupting their cell membranes, often with detergent or other chaotropic agent,(s ... Crude cell lysates are routinely produced in biochemistry and cell biology laboratories during the process of protein purification, although purified cellular organelles can also be retrieved from the solution ... After a crude lysate has been generated, the first step in processing a crude lysate is often ultracentrifugation, which separates the solution into distinct bands containing organelles, membrane lipids ...