Immunoprecipitation

Immunoprecipitation (IP) is the technique of precipitating a protein antigen out of solution using an antibody that specifically binds to that particular protein. This process can be used to isolate and concentrate a particular protein from a sample containing many thousands of different proteins. Immunoprecipitation requires that the antibody be coupled to a solid substrate at some point in the procedure.

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Other articles related to "immunoprecipitation":

Methods of Epigenomics - Histone Modification Assays - ChIP-Chip and ChIP-Seq
... modification was first detected on a genome wide level through the coupling of chromatin immunoprecipitation (ChIP) technology with DNA microarrays, termed ChIP-Chip (Barski et al ... factor or enhancer protein through chromatin immunoprecipitation, the proteins of interest are the modified histones themselves ... Following immunoprecipitation, the DNA is purified from the histones, amplified via PCR and labeled with a fluorescent tag (e.g ...
Immunoprecipitation - Protocol - Steps
... Lyse cells and prepare sample for immunoprecipitation ... Pre-clear the sample by passing the sample over beads alone or bound to an irrelevant antibody to soak up any proteins that non-specifically bind to the IP components ...
Methods - Methylated DNA Immunoprecipitation (MeDIP)
... adequate resolution, improving the efficiency of the downstream step in immunoprecipitation, and reducing fragment-length effects or biases ... The classical immunoprecipitation technique is then applied magnetic beads conjugated to anti-mouse-IgG are used to bind the anti-5mC antibodies, and unbound ...
Chromatin Immunoprecipitation
... Chromatin Immunoprecipitation (ChIP) is a type of immunoprecipitation experimental technique used to investigate the interaction between proteins and DNA in the cell ...
DNA Methylation - Detection
... Methylated DNA immunoprecipitation (MeDIP), analogous to chromatin immunoprecipitation, immunoprecipitation is used to isolate methylated DNA fragments for input into DNA detection methods such as DNA microarrays (Me ...