Immobilized Enzyme

An immobilized enzyme is an enzyme that is attached to an inert, insoluble material such as calcium alginate (produced by reacting a mixture of sodium alginate solution and enzyme solution with calcium chloride). This can provide increased resistance to changes in conditions such as pH or temperature. It also allows enzymes to be held in place throughout the reaction, following which they are easily separated from the products and may be used again - a far more efficient process and so is widely used in industry for enzyme catalysed reactions. An alternative to enzyme immobilization is whole cell immobilization.

Read more about Immobilized EnzymeCommercial Use, Immobilization of An Enzyme

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Immobilized Enzyme - Immobilization of An Enzyme
... There are three different ways by which one can immobilise an enzyme, which are the following, listed in order of effectiveness Adsorption on glass ... As adsorption is not a chemical reaction, the active site of the immobilized enzyme may be blocked by the matrix or bead, greatly reducing the activity of the enzyme ... Entrapment The enzyme is trapped in insoluble beads or microspheres, such as calcium alginate beads ...