Htert

Some articles on htert:

Telomerase Reverse Transcriptase - Regulation of HTERT
... The hTERT gene, located on chromosome 5, consists of 16 exons and 15 introns spanning 35kb ... The core promoter of hTERT includes 330 base pairs upstream of the translation start site (ATG), as well as 37 base pairs of exon 2 of the hTERT gene ... The hTERT promoter is GC-rich and lacks TATA and CAAT boxes but contains many sites for several transcription factors giving indication of a high level of regulation ...
Telomerase Reverse Transcriptase - Medical Implications - Gene Therapy
... The hTERT gene has become a main focus for gene therapy involving cancer due to its expression in tumor cells but not somatic adult cells ... One method is to prevent the translation of hTERT mRNA through the introduction of siRNA which are complimentary sequences that bind to the mRNA preventing processing of the gene post ... not completely eliminate telomerase activity, but it does lower telomerase activity and levels of hTERT mRNA seen in the cytoplasm ...
Telomerase Reverse Transcriptase - Medical Implications - IPS Cells
... By using immortalized somatic cells (differentiated cells with hTERT up regulated), iPS cell reprogramming was increased by twenty fold compared to reprogramming using mortal cells ... The reactivation of hTERT, and subsequently telomerase, in human iPS cells has been used as an indication of pluripotency and reprogramming to an ES (embryonic stem) cell-like state ... Reprogrammed cells which do not express sufficient hTERT levels enter a quiescent state following a number of replications depending on the length of ...
Telomerase Reverse Transcriptase - Stem Cells - HTERT in Stem Cells
... hTERT is often up-regulated in cells that divide rapidly, including both embryonic stem cells and adult stem cells ... time, not contribute to aberrant telomere elongation High expression of hTERT is also often used as a landmark for pluripotency and multipotency state ... Over-expression of hTERT was found to immortalize certain cell types as well as impart different interesting properties to different stem cells ...
Telomerase Reverse Transcriptase - Stem Cells - Immortalization
... hTERT immortalizes various normal cells in culture, thereby endowing the self-renewal properties of stem cells to non-stem cell cultures ... of non-stem cells can be achieved, one of which is via the introduction of hTERT into the cells ... protein that helps form the telomerase assembly, but does not express hTERT ...