Gateway Cloning

Some articles on gateway cloning, gateway, cloning:

Gateway Technology - Basic Steps
... The first step in Gateway cloning is the preparation of a Gateway Entry clone ... Entry clones are often made in two steps 1) “Gateway attB1, and attB2” sequences are added to the 5’, and 3’ end of a gene fragment, respectively, using gene specific PCR primers and PCR-amplification 2) the ... insertion of the att-B-sequence-containing PCR product into the att P recombination sites in the Gateway Donor vector ...
Gateway Technology
... The Gateway cloning System, invented and commercialized by Invitrogen since the late 1990s, is a molecular biology method that enables researchers to efficiently transfer DNA-fragments between plasmids using a ... Gateway Cloning Technique allows transfer of DNA fragments between different cloning vectors while maintaining the reading frame ... Using Gateway, one can clone or subclone DNA segments for functional analysis ...

Famous quotes containing the words cloning and/or gateway:

    Language is as real, as tangible, in our lives as streets, pipelines, telephone switchboards, microwaves, radioactivity, cloning laboratories, nuclear power stations.
    Adrienne Rich (b. 1929)

    The whole fauna of human fantasies, their marine vegetation, drifts and luxuriates in the dimly lit zones of human activity, as though plaiting thick tresses of darkness. Here, too, appear the lighthouses of the mind, with their outward resemblance to less pure symbols. The gateway to mystery swings open at the touch of human weakness and we have entered the realms of darkness. One false step, one slurred syllable together reveal a man’s thoughts.
    Louis Aragon (1897–1982)