DNA Polymerase III

Some articles on polymerase, dna polymerase, dna, polymerases, dna polymerase iii:

... A polymerase is an enzyme whose central biological function is the synthesis of polymers of nucleic acids ... DNA polymerase and RNA polymerase are used to assemble DNA and RNA molecules, respectively, generally by copying a DNA or RNA template strand using base-pairing interactions ... for the generation of other biopolymers are not also referred to as polymerases ...
Prokaryotic DNA Replication - Elongation
... Once priming is complete, DNA polymerase III holoenzyme is loaded into the DNA and replication begins ... The catalytic mechanism of DNA polymerase III involves the use of two metal ions in the active site, and a region in the active site that can discriminate between deoxyribonucleotides and ribonucleotides ... This hydrolysis drives DNA synthesis to completion ...
TOPO Cloning - Technique
... The linear vector DNA already has the topoisomerase enzyme covalently attached to both of its strands' free 3' ends ... types of vectors are used for cloning fragments amplified by either Taq or Pfu polymerase as Taq polymerase (unlike Pfu) leaves an extra "A" nucleotide at the 3'end during amplification ... of adenine (A) and thymine (T) (complementary basepairs) on different DNA fragments to hybridize and, in the presence of ligase or topoisomerase, become ligated together ...
... nucleic acid chain elongation by binding to the polymerase, thus stopping RNA polymerase activity inside a cell ... it inhibits the assembly of the RNA polymerase II transcription complex and DNA polymerase III transcription ...
DNA Clamp - Structure - Bacterial
... DNA polymerase III subunit beta Crystallographic structure of the dimeric DNA polymerase beta subunit from E. Chromosome MG1655 3.88 - 3.88 Mb The beta clamp is a specific DNA clamp and a subunit of the DNA polymerase III holoenzyme found in bacteria ... Two beta subunits are assembled around the DNA by the gamma subunit and ATP hydrolysis this assembly is called the pre-initiation complex ...

Famous quotes containing the words iii and/or dna:

    The army is the true nobility of our country.
    —Napoleon Bonaparte III (1808–1873)

    Here [in London, history] ... seemed the very fabric of things, as if the city were a single growth of stone and brick, uncounted strata of message and meaning, age upon age, generated over the centuries to the dictates of some now all-but-unreadable DNA of commerce and empire.
    William Gibson (b. 1948)