The core principle behind microarrays is hybridization between two DNA strands, the property of complementary nucleic acid sequences to specifically pair with each other by forming hydrogen bonds between complementary nucleotide base pairs. A high number of complementary base pairs in a nucleotide sequence means tighter non-covalent bonding between the two strands. After washing off of non-specific bonding sequences, only strongly paired strands will remain hybridized. Fluorescently labeled target sequences that bind to a probe sequence generate a signal that depends on the hybridization conditions (such as temperature), and washing after hybridization. Total strength of the signal, from a spot (feature), depends upon the amount of target sample binding to the probes present on that spot. Microarrays use relative quantization in which the intensity of a feature is compared to the intensity of the same feature under a different condition, and the identity of the feature is known by its position.
Read more about this topic: DNA Microarray
Other articles related to "principle, principles":
... Principles derive from tradition, such as the concept of matching ... Principle of regularity Regularity can be defined as conformity to enforced rules and laws ... Principle of consistency This principle states that when a business has once fixed a method for the accounting treatment of an item, it will enter in ...
... The Franck–Condon principle is a rule in spectroscopy and quantum chemistry that explains the intensity of vibronic transitions ... The principle states that during an electronic transition, a change from one vibrational energy level to another will be more likely to happen if the two vibrational wave functions overlap more ...
... …The first aspect of this principle is that of becoming, where it appears as the law of causality and is applicable only to changes ... from representations of intuitive perception, and here the principle of sufficient reason states that, if certain premises are given, the conclusion must follow ... The third aspect of the principle is concerned with being in space and time, and shows that the existence of one relation inevitably implies the other, thus that the equality of the angles of a triangle ...
... to freedom of speech may follow the "harm principle" or the "offense principle", for example in the case of pornography, or hate speech ... Mill also introduced what is known as the harm principle, in placing the following limitation on free expression "the only purpose for which power can be rightfully exercised over any member of a civilized community ... and that it is probably a necessary means to that end." Hence Feinberg argues that the harm principle sets the bar too high and that some forms of expression can be ...
Famous quotes containing the word principle:
“A certain secret jealousy of the British Minister is always lurking in the breast of every American Senator, if he is truly democratic; for democracy, rightly understood, is the government of the people, by the people, for the benefit of Senators, and there is always a danger that the British Minister may not understand this political principle as he should.”
—Henry Brooks Adams (18381918)
“No habit or quality is more easily acquired than hypocrisy, nor any thing sooner learned than to deny the sentiments of our hearts and the principle we act from: but the seeds of every passion are innate to us, and nobody comes into the world without them.”
—Bernard Mandeville (16701733)
“Help thyself: then everyone will help thee too. Principle of Christian charity.”
—Friedrich Nietzsche (18441900)