Digital Polymerase Chain Reaction

Digital Polymerase Chain Reaction (digital PCR, DigitalPCR, dPCR, or dePCR) is a refinement of conventional polymerase chain reaction methods that can be used to directly quantify and clonally amplify nucleic acids including DNA, cDNA or RNA. The key difference between dPCR and traditional PCR lies in the method of measuring nucleic acids amounts, with the former being a more precise method than PCR. PCR carries out one reaction per single sample. dPCR also carries out a single reaction within a sample, however the sample is separated into a large number of partitions and the reaction is carried out in each partition individually. This separation allows a more reliable collection and sensitive measurement of nucleic acid amounts. The method has been demonstrated as useful for studying variations in gene sequences - such as copy number variants and point mutations - and it is routinely used for clonal amplification of samples for "next-generation sequencing."

Read more about Digital Polymerase Chain ReactionPCR Basics, DPCR Working Principle, Development

Other articles related to "digital polymerase chain reaction, digital, reaction, polymerase, polymerases, chain":

Digital Polymerase Chain Reaction - Development
... any inappropriate external links The physically partitioned, macro-chamber digital PCR concept was conceived in 1992 by Sykes et al ... The concepts of electrowetting and digital microfluidics were further introduced (Brown) as a means of manipulating nano fluid volumes and carrying out Digital PCR ... Digital PCR has been shown to be a promising surveillance tool for illnesses such as cancer, and as a vital front end to determining genomic content, including sequencing the human genome ...
TOPO Cloning - Technique
... This reaction proceeds efficiently when this solution is incubated at room temperature with required salt ... cloning fragments amplified by either Taq or Pfu polymerase as Taq polymerase (unlike Pfu) leaves an extra "A" nucleotide at the 3'end during amplification ... The insert is created by PCR using Taq DNA polymerase, a polymerase that lacks 3' to 5' proofreading activity and with a high probability adds a single, 3'-adenine overhang to each end of the PCR product ...
... A polymerase is an enzyme whose central biological function is the synthesis of polymers of nucleic acids ... DNA polymerase and RNA polymerase are used to assemble DNA and RNA molecules, respectively, generally by copying a DNA or RNA template strand using base-pairing interactions ... for the generation of other biopolymers are not also referred to as polymerases ...
... Streptolydigin is an antibiotic which works by blocking nucleic acid chain elongation by binding to the polymerase, thus stopping RNA polymerase activity inside a cell ... it inhibits the assembly of the RNA polymerase II transcription complex and DNA polymerase III transcription ...
T7 DNA Polymerase
... The T7 DNA polymerase of the T7 bacteriophage is a DNA-dependent DNA polymerase responsible for the fast rate of T7 phage DNA replication in vivo ... The polymerase consists of a 11 complex of the viral T7 gene 5 protein (80kDA) and the E ... This polymerase is unique due to its considerable processivity, or ability to stay on DNA for a greater than average number of base pairs ...

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