AP Endonucleases

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AP Endonucleases

Apurinic/apyrimidinic (AP) endonuclease (BRENDA = 4.2.99.18) is an enzyme that is involved in the DNA base excision repair pathway (BER). Its main role in the repair of damaged or mismatched nucleotides in DNA is to create a nick in the phosphodiester backbone of the AP site created when DNA glycosylase removes the damaged base. There are four types of AP endonucleases that have been classified according to their sites of incision. Class I and class II AP endonucleases incise DNA at the phosphate groups 3´ and 5´ to the baseless site leaving 3´-OH and 5´-phosphate termini. Class III and class IV AP endonucleases also cleave DNA at the phosphate groups 3´ and 5´to the baseless site, but they generate a 3´-phosphate and a 5´-OH. Human AP Endonuclease (APE1), like most AP endonucleases, is of class II and requires an Mg2+ in its active site in order to carry out its role in base excision repair.

Read more about AP EndonucleasesStructure of APE1, Mechanism, Inhibition of APE1, APE1 As Chemopreventive Target

Other articles related to "ap, endonuclease, ap endonucleases":

AP Endonuclease
... Apurinic/apyrimidinic (AP) endonuclease (BRENDA = 4.2.99.18) is an enzyme that is involved in the DNA base excision repair pathway (BER) ... nucleotides in DNA is to create a nick in the phosphodiester backbone of the AP site created when DNA glycosylase removes the damaged base ... There are four types of AP endonucleases that have been classified according to their sites of incision ...
Proteins Involved in Base Excision Repair - AP Endonucleases
... The AP endonucleases cleave an AP site to yield a 3' hydroxyl adjacent to a 5' deoxyribosephosphate (dRP) ... AP endonucleases are divided into two families based on their homology to the ancestral bacterial AP endonucleaes endonuclease IV and exonuclease III ... In humans, two AP endonucleases, APEX1 and APEX2, have been identified ...
AP Endonucleases - APE1 As Chemopreventive Target
... so that the enzymes involved later in the BER pathway can recognize the AP-site, it also has a redox function that helps activate other enzymes involved in DNA repair ...