Antimicrobial Peptides - Mode of Action

Mode of Action

Several methods have been used to determine the mechanisms of antimicrobial peptide activity. In particular, solid-state NMR studies have provided an atomic-level resolution explanation of membrane disruption by antimicrobial peptides.

Methods Applications
Microscopy to visualize the effects of antimicrobial peptides on microbial cells.
Fluorescent dyes to measure antimicrobial peptides to permeabilize membrane vesicles.
Ion channel formation to assess the formation and stability of an antimicrobial-peptide-induced pore.
Circular dichroism and orientated circular dichroism to measure the orientation and secondary structure of an antimicrobial peptide bound to a lipid bilayer
Dual Polarization Interferometry to measure the different mechanisms of antimocrobial peptides
Solid-state NMR spectroscopy to measure the secondary structure, orientation and penetration of antimicrobial peptides into lipid bilayers in the biologically relevant LIQUID-CRYSTALLINE STATE
Neutron and X-ray diffraction to measure the diffraction patterns of peptide-induced pores within membranes in oriented multilayers or liquids

Read more about this topic:  Antimicrobial Peptides

Other articles related to "mode of action":

Mode Of Action

A mode of action (MoA) describes a functional or anatomical change, at the cellular level, resulting from the exposure of a living organism to a substance. In comparison, a mechanism of action (MOA) describes such changes at the molecular level.

A mode of action is important in classifying chemicals as it represents an intermediate level of complexity in between molecular mechanisms and physiological outcomes, especially when the exact molecular target has not yet been elucidated or is subject to debate. A mechanism of action of a chemical could be "binding to DNA" while its broader mode of action would be "transcriptional regulation". However, there is no clear consensus and the term mode of action is often used, especially in the study of pesticides, to also describe molecular mechanisms such as action on specific nuclear receptors or enzymes.

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