Antimicrobial Peptides - Mode of Action

Mode of Action

Several methods have been used to determine the mechanisms of antimicrobial peptide activity. In particular, solid-state NMR studies have provided an atomic-level resolution explanation of membrane disruption by antimicrobial peptides.

Methods Applications
Microscopy to visualize the effects of antimicrobial peptides on microbial cells.
Fluorescent dyes to measure antimicrobial peptides to permeabilize membrane vesicles.
Ion channel formation to assess the formation and stability of an antimicrobial-peptide-induced pore.
Circular dichroism and orientated circular dichroism to measure the orientation and secondary structure of an antimicrobial peptide bound to a lipid bilayer
Dual Polarization Interferometry to measure the different mechanisms of antimocrobial peptides
Solid-state NMR spectroscopy to measure the secondary structure, orientation and penetration of antimicrobial peptides into lipid bilayers in the biologically relevant LIQUID-CRYSTALLINE STATE
Neutron and X-ray diffraction to measure the diffraction patterns of peptide-induced pores within membranes in oriented multilayers or liquids

Read more about this topic:  Antimicrobial Peptides

Other articles related to "mode of action":

Mode Of Action

A mode of action (MoA) describes a functional or anatomical change, at the cellular level, resulting from the exposure of a living organism to a substance. In comparison, a mechanism of action (MOA) describes such changes at the molecular level.

A mode of action is important in classifying chemicals as it represents an intermediate level of complexity in between molecular mechanisms and physiological outcomes, especially when the exact molecular target has not yet been elucidated or is subject to debate. A mechanism of action of a chemical could be "binding to DNA" while its broader mode of action would be "transcriptional regulation". However, there is no clear consensus and the term mode of action is often used, especially in the study of pesticides, to also describe molecular mechanisms such as action on specific nuclear receptors or enzymes.


Hyblaea Puera Nucleopolyhedrovirus (HpNPV) - Mode of Action
... The discovery of virus disease on H ... puera was a major break through in the teak defoliator management research ...
Pore-forming Toxin - Types of PFTs - Beta-pore-forming Toxins - Mode of Action
... A structure of the Vibrio cholerae Cytolysin in the pore form is also heptameric, however Staphylococcus aureus gamma-hemolysin reveals an octomeric pore, consequently with a 16-strand 'stalk' ... The Panton-Valentine Leucocidin S structure shows a highly related structure, but in its soluble monomeric state ...

Famous quotes containing the words mode of, action and/or mode:

    Sight is the least sensual of all the senses. And we strain ourselves to see, see, see—everything, everything through the eye, in one mode of objective curiosity.
    —D.H. (David Herbert)

    Mountains are to the rest of the body of the earth, what violent muscular action is to the body of man. The muscles and tendons of its anatomy are, in the mountain, brought out with force and convulsive energy, full of expression, passion, and strength.
    John Ruskin (1819–1900)

    The method of scientific investigation is nothing but the expression of the necessary mode of working of the human mind. It is simply the mode in which all phenomena are reasoned about, rendered precise and exact.
    Thomas Henry Huxley (1825–95)