ABI Solid Sequencing

ABI Solid Sequencing

SOLiD (Sequencing by Oligonucleotide Ligation and Detection) is a next-generation DNA sequencing technology developed by Life Technologies and has been commercially available since 2008. This next generation technology generates hundreds of millions to billions of small sequence reads at one time.

This method should not be confused with "sequencing by synthesis," a principle used by Roche-454 pyrosequencing (introduced in 2005, generating millions of 200-400bp reads in 2009), and the Solexa system (now owned by Illuimina) (introduced in 2006, generating hundreds of millions of 50-100bp reads in 2009)

These methods have reduced the cost from $0.01/base in 2004 to nearly $0.0001/base in 2006 and increased the sequencing capacity from 1,000,000 bases/machine/day in 2004 to more than 5,000,000,000 bases/machine/day in 2009. Over 30 publications exist describing its use first for nucleosome positioning from Valouev et al., transcriptional profiling or strand sensitive RNA-Seq with Cloonan et al., single cell transcriptional profiling with Tang et al. and ultimately human resequencing with McKernan et al.

Read more about ABI Solid SequencingChemistry, Throughput and Accuracy, Applications

Other articles related to "abi solid sequencing, sequencing":

ABI Solid Sequencing - Applications
... Transcriptomics by next gen sequencing will mean these barriers no longer hold true ... Next gen sequencing can also be applied in this area ...

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